Journal: IBRO Neuroscience Reports
Article Title: Effect of BMSCs overexpressing intelectin-1 on angiogenesis in rats with cerebral infarction
doi: 10.1016/j.ibneur.2025.03.012
Figure Lengend Snippet: BMSCs overexpressing Itln-1 reduced infarct volume and increased the regional cerebral blood flow (Each group n = 8):(A) CI volume in each group by TTC staining. (B) The expression of Itln-1 mRNA in the cerebral ischemic penumbra. (C) The regional cerebral blood flow (Perfusion Unit) measured by the Doppler laser bloodstream detector.(D) Immunohistochemistry of itlin-1 and GFP. **** P < 0.0001.
Article Snippet: One week after MCAO,the skull was secured in a stereotactic frame,and a small enclosed cranial window was created on the left hemisphere to measure local cerebral blood flow (lCBF) using a Doppler laser blood flow detector (Peri Flux 5000, Perimed AB,Beijing).The standard cellular nucleus appeared blue,while the nucleus of apoptotic cells showed a deeper color due to dye uptake and a duller fluorescence from the DNA fluorochromes.
Techniques: Staining, Expressing, Immunohistochemistry